An approach to searching for specific proteins associated with active genes in hen oviduct.
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چکیده
منابع مشابه
Immunoreactive avidin in the hen oviduct mucosa.
diameter)in the gland and small(200 to 700 nm) in the epithelium. Epithelial cells containing secretory granules had a strong resemblance to those of the protodifferentiated gland cells appearing in the magnum of chicks pretreated with hormones. No avidin was observed in any epithelial goblet cells or ciliated cells. The findings paralleled those obtained by biotinylatedenzymes affinity cytoche...
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An enzymatic reaction has been identified in the isthmus of hen oviduct in which sulfate is transferred from 3’-phosphoadenosine 5’-phosphosulfate to mucopolysaccharide acceptors (1). From studies of the reaction with monosaccharides and oligosaccharides as model acceptors (‘2) it was concluded that one or more enzymes in the preparations employed catalyze a direct transfer of sulfate from PAPS...
متن کاملGlucocorticoid binding in the hen oviduct.
[(3)H]Triamcinolone acetonide (15nm) was incubated with cytosol (150000g fraction) prepared from oviducts of egg-laying hens. The extent of steroid binding, as determined by charcoal assays, was greatest between 2-4h at 4 degrees C. A similar time curve was obtained when cytosol preparations were first fractionated with (NH(4))(2)SO(4) before labelling. The addition of 10mm-Na(2)MoO(4) or 10mm-...
متن کاملInteraction of oestrogen and progesterone receptors with specific subfractions of laying-hen oviduct chromatin.
Salt (NaCl)-extracted nuclear oestrogen receptor from hen oviduct was incubated with salt-depleted oviduct chromatin and dialysed to low salt. The oestrogen receptor (re)associated with chromatin to form a 13-14S-sedimenting fraction, as found in 'native' chromatin, and saturation of this interaction was obtained for very low receptor concentrations (approx. 0.04 nM). Similarly, purified proges...
متن کاملHen oviduct signal peptidase is an integral membrane protein.
Membrane preparations from rough endoplasmic reticulum of hen oviduct resemble those of dog pancreas in their capacity to translocate nascent secretory proteins into membrane vesicles present during cell-free protein synthesis. As with the dog membranes, the precursor form of human placental lactogen is transported into the vesicles and processed to the native secretory form by an associated "s...
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ژورنال
عنوان ژورنال: Endocrinologia Japonica
سال: 1984
ISSN: 0013-7219,2185-6370
DOI: 10.1507/endocrj1954.31.509